EASY-nLC™ 1200 System
Manufacturer – Thermo Fisher Scientific
Description: EASY-nLC™ 1200 System is a high-performance nano-flow UHPLC designed for nano-LC-MS workflows, offering up to 1200 bar operating pressure and exceptional flow and gradient precision. It provides robust, maintenance-free operation with intuitive software control for reproducible, high-throughput proteomics analysis.
Technical Specifications:
Pressure range: 0 – 1200 bar
Flow range (gradient): 20 – 2000 nL/min (recommended 100 – 1000 nL/min)
Flow range (loading/equilibration): ≤ 25 µL/min
Retention time reproducibility: 0.1 – 0.4 % RSD within recommended range
Pick-up volume range: 0.10 – 18.00 µL (20 µL loop default)
Injection reproducibility: ≤ 0.2 % RSD (at 5 µL pick-up); ≤ 3.0 % RSD (at 0.1 µL pick-up)
Gradient delay volume: < 1 µL (from mixing tee to venting tee)
Solvent compatibility: ≤ 95 % acetonitrile in water; wetted parts include fused silica, PEEK™, 316 stainless steel, zirconium dioxide
Sample tray formats: 6×8 HPLC vials; 1×96 or 1×384 MTP + 6 vials; 2×48 or 4×96 PCR strips + 6 vials
Autosampler cooling: 5 °C minimum, up to 20 °C below ambient
Operating conditions: Ambient 5 – 30 °C; 20 – 80 % relative humidity
Dimensions (H×W×D): 45 × 36 × 38 cm (18″ × 14″ × 15″)
Weight: 37 kg (≈ 82 lb)
Power requirements: 120 / 230 Vac, 50/60 Hz, 250 W
Key Features:
• Operates up to 1200 bar for high-resolution separations and faster runs
• Maintenance-free ceramic valves for maximum robustness and uptime
• nanoViper™ tool-free connections ensure leak-free, low-dead-volume fittings
• Dual in-line flow sensors for superior gradient accuracy
• Fully integrated with Thermo Scientific™ Xcalibur™ software for unified LC-MS control
• Intelligent maintenance and diagnostic routines for system reliability
• Compact benchtop design with touchscreen interface for ease of use
Applications: Designed for high-sensitivity nano-flow liquid chromatography in proteomics and biomolecular LC-MS/MS applications, enabling deep proteome coverage and reproducible, high-throughput analysis.